Lastly, ELISA was employed for the measurements of mouse IgG using the system consisting of the bento box and the polypropylene device. We confirmed, through real-time observation of the device, that accurate flow control in the designed ELISA procedure was executed.
Next, we evaluated the injection-molded device through multi-step fluid control. Therefore, the controllability of the bento box was demonstrated. The limit of detection (LOD) using the bento box was 0.759 ng ml −1. It was confirmed that the results of the bento box were consistent with those of the servo-controlled device driver. Here, we first executed an ELISA using a system consisting of the bento box and a device made of polydimethylsiloxane (PDMS) and compared it with a servo-controlled device driver. However, when Akihiro, the school giant, comes to his rescue, Jun finds out that looks can be deceiving, first loves are. He wants to play soccer, but he sucks at sports. Hes a third year in high school, but he looks like a first year. A BL Comic by Myssdark and EAB Jun Watanabe is your average outcast. The developed device can automatically execute an enzyme-linked immunosorbent assay (ELISA) process under a steady rotating condition because it was designed based on the principle of CLOCK, which we previously presented. Bento- 3, Episode 9 of My Gentle Giant in WEBTOON. It has a simple structure and is the size of a bento box, that is, 150 × 150 × 100 ( W × D × H) mm 3. The bento box consists of a cheap DC motor and an Arduino microcontroller.
UPGRADE FROM BENTO 3 TO BENTO 4 DRIVER
In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding.